6/14/2023 0 Comments Download free organellesFurther, we demonstrate multiple strategies for controlled cargo release from condensates to switch cells between functional states. By relocalizing up to ninety percent of a targeted enzymes to synthetic condensates, we efficiently control cellular behaviors, including proliferation, division, and cytoskeletal organization. We engineer and express a disordered protein scaffold to assemble micron size condensates and recruit endogenous clients via genomic tagging with high-affinity dimerization motifs. We report a synthetic membraneless organelle platform to control endogenous cellular activities through sequestration and insulation of native proteins. Inspired by this functionality, we sought to build designer compartments that function as hubs to regulate the flow of information through cellular control systems. Subcellular compartmentalization of macromolecules increases flux and prevents inhibitory interactions to control biochemical reactions.
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